3D structures to the domains

We initiate the process using these sequences as input seed for Hmmer, then, after some iterations we obtain this seed .

_images/NterminalDomain.png

The resulting complete sequences from uniprot can be download from here these sequences

The taxonomy of the search results are:

_images/taxTrimer.png

The domains of the search results are:

_images/domainTrimer.png _images/domtrimer2.png _images/domtrimer3.png

We made the same search but using only PDB databank instead of uniprot, these are the results

_images/pdbs.png

The taxonomy of the search results are:

_images/taxpdb.png

The domains of the search results are:

_images/dompdb.png

Using pymol, we can observe that all the pdbs are trimer, the pdbs are here

1yu0 2c3f 2dp5 2iou 2yw0 6x3m 1yu1 1yu2 1yu3 1yu4 2yvv 3eka

Considering the species we have:

ARCHEA

Interpro L0KWD4, the alpha fold model as a trimer is in this link

_images/L0KWD4_trimerDomain.png _images/L0KWD4_trimerbfactor.png _images/L0KWD4_trimer.png

EUKARIOT

Interpro B9TE92, the alpha fold model as a trimer is in this link

_images/B9TE92_trimerDomain.png _images/B9TE92_trimerbfactor.png _images/B9TE92_trimer.png

BACTERIA

The 128 bacterias present in this cluster have an AF model as a monomer, we took three small sequences and model them as trimers.

A0A0F2JLV1

A0A1H4DFS6.fasta A0A4S4BSN2.fasta

We also try to observe the amino acids present in all these sequences:

_images/bacteriaAA.png

VIRUS

The 212 bacterias present in this cluster do not have an AF model as a monomer nor as trimer. We took two sequences and model them as trimers.

Interpro C7F4B3

_images/C7F4B3_trimer.png _images/C7F4B3_trimerDomain.png _images/C7F4B3_trimerbfactor.png

Interpro B5U373

_images/B5U373_trimer.png _images/B5U373_trimerbfactor.png _images/B5U373_trimerDomain.png

We also try to observe the amino acids present in all these sequences:

_images/virusAA.png

A SECOND DOMAIN

Literature

Seed

_images/Logo2ndDomain.png _images/taxDom2.png _images/Domain2_dom.png _images/Domain2_dom2.png _images/Domain2_dom3.png

K6SYF0

Monomer AF model AF-K6SYF0-F1-model_v4.pdb

Pymol Sesion K6SYF0.pse

_images/K6SYF0_bfactor.png _images/K6SYF0.png

A THIRD DOMAIN

Model Seed

_images/LogoDomain3.png _images/taxDom3.png _images/Domain3_dom.png _images/Domain3_dom2.png _images/Domain3_dom3.png _images/Domain3_dom3.png

E9I2F2 (EUK)

_images/E9I2F2.png _images/E9I2F2bfactor.png

Monomer AF model E9I2F2

Pymol Sesion E9I2F2.pse

Trimer AF model E9I2F2E9I2F2trimer.pdb